abstract：:In this review we discuss the yeast as a paradigm for the study of aging. The budding yeast Saccharomyces cerevisiae, which can proliferate in both haploid and diploid states, has been used extensively in aging research. The budding yeast divides asymmetrically to form a 'mother' cell and a bud. Two major approaches, ...

journal_title：Mechanisms of ageing and development

authors： Gershon H,Gershon D

更新日期：2000-12-01 00:00:00

abstract：:Telomeres are supposed to play a role in cellular aging and might contribute to the genetic background of human aging and longevity. During the past few years telomere length has been measured in various human tissues. However, very little is known about the individual telomere loss in different tissues from the same ...

journal_title：Mechanisms of ageing and development

authors： Friedrich U,Griese E,Schwab M,Fritz P,Thon K,Klotz U

更新日期：2000-11-15 00:00:00

abstract：:It is generally accepted that free-radical induced blood lipid peroxidation and especially peroxidized LDL play a central role in the pathogenesis of atherosclerosis and related cardiovascular disease. Moreover, recent research highlights the key contribution of apolipoprotein B (apo B) to atherogenesis as the main in...

journal_title：Mechanisms of ageing and development

authors： Ramírez-Boscá A,Soler A,Carrión MA,Díaz-Alperi J,Bernd A,Quintanilla C,Quintanilla Almagro E,Miquel J

更新日期：2000-10-20 00:00:00

abstract：:At least five different transgenic approaches have been applied to the study of Drosophila aging. There are two single component systems: transgenes with native (normal) promoters and transgenes with heterologous promoters; as well as three binary systems: 'GAL4/UAS', 'FLP-out' and 'tet-on'. These approaches vary in a...

journal_title：Mechanisms of ageing and development

authors： Tower J

更新日期：2000-09-01 00:00:00

abstract：:This is the first in a series of articles in which we intend to critically review some of the currently used models in gerontology research and evaluate their contribution to advancing our understanding of the phenomenon of senescence. The major theories of aging are considered. We discuss what makes a model useful in...

journal_title：Mechanisms of ageing and development

authors： Gershon H,Gershon D

更新日期：2000-08-15 00:00:00

abstract：:Active vitamin D drugs are used for the treatment of osteoporosis in a number of countries, including Japan. However, their use is controversial. Here, we briefly discuss two issues that are important for understanding the role of active vitamin D (rather than plain vitamin D) in the treatment of osteoporosis: (1) whe...

journal_title：Mechanisms of ageing and development

authors： Ikeda K,Ogata E

更新日期：2000-07-31 00:00:00

abstract：:Calorie restriction (CR) extends life span and retards many age-related cellular and molecular changes in laboratory rodents. However, neither the breadth of its effects, its underlying mechanisms, nor the limits of its action is fully understood. Expression levels of 588 genes in livers from 3- and 24-month-old ad li...

journal_title：Mechanisms of ageing and development

authors： Han E,Hilsenbeck SG,Richardson A,Nelson JF

更新日期：2000-06-20 00:00:00

abstract：:Aflatoxin B1(AFB1)-glutathione(GSH) conjugation is the major pathway for the detoxification of aflatoxin metabolites. This reaction is catalysed by GSH S-transferase (GST) and play a major role in modulation of AFB1 adduct formation to nuclear DNA. Changes recorded in hepatic GST activity during development of rats ca...

journal_title：Mechanisms of ageing and development

authors： Allameh A,Farahani M,Zarghi A

更新日期：2000-05-18 00:00:00

abstract：:The data concerning the effects of age on the brainstem are scarce and few works are devoted to the human vestibular nuclear complex. The study of the effects of aging in the vestibular nuclei could have clinical interest due to the high prevalence of balance control and gait problems in the elderly. We have used in t...

journal_title：Mechanisms of ageing and development

authors： Alvarez JC,Díaz C,Suárez C,Fernández JA,González del Rey C,Navarro A,Tolivia J

更新日期：2000-04-14 00:00:00

abstract：:The present study examined the effect of a 40% reduction in caloric intake (CR) versus ad libitum (AL) feeding on retinal aging. CR- and AL-fed Brown Norway (BN) rats were obtained at 12, 24 and 30 months of age from the National Center for Toxicological Research (NCTR). Age-dependent declines in outer nuclear layer (...

journal_title：Mechanisms of ageing and development

authors： Obin M,Pike A,Halbleib M,Lipman R,Taylor A,Bronson R

更新日期：2000-03-13 00:00:00

abstract：:The endothelin-1 (ET-1) concentrations were measured by RIA in the media of confluent monolayer cultures of rat articular chondrocyte (RAC) exposed to fetal calf serum (FCS) and several growth factors and cytokines. The cells were obtained from 1- and 18-month-old rats. First passage cells were starved in Dulbecco's m...

journal_title：Mechanisms of ageing and development

authors： Messai H,Khatib AM,Lebrun G,Aubin P,Florina M,Jean F,Mitrovic DR

更新日期：2000-02-22 00:00:00

abstract：:Lymphatic lipid transport in the intestine of adult and ageing rats was compared. Adult (8-10 months old) and old (24-26 months old) male Wistar rats were cannulated into the mesenteric lymph under ethrane anesthesia. On the following day, lipid emulsion containing 35.4 mg/h of olive oil was infused intraduodenally fo...

journal_title：Mechanisms of ageing and development

authors： Hayashi H,Sato Y,Kanai S,Masuda M,Ohta M,Funakoshi A,Nagao K,Imaizumi K,Miyasaka K

更新日期：2000-02-15 00:00:00

abstract：:We used a fluorescence differential display--PCR (FDD-PCR) technique to analyze the genes expressed in mouse kidneys collected at nine different developmental stages ranging from 3 days to 15 months after birth. We found ten genes that were age-dependent and differentially-expressed in the kidneys during our experimen...

journal_title：Mechanisms of ageing and development

authors： Iida R,Yasuda T,Aoyama M,Tsubota E,Matsuki T,Kishi K

更新日期：2000-02-07 00:00:00

abstract：:Oxidative stress may contribute to the cellular alterations, which occur as the result of aging, and the nervous system is particularly vulnerable to aging associated oxidative injury. The multicatalytic proteasome (MCP) is responsible for the majority of protein degradation and is sensitive to oxidative stress. To de...

journal_title：Mechanisms of ageing and development

authors： Keller JN,Hanni KB,Markesbery WR

更新日期：2000-01-24 00:00:00

abstract：:To test the hypothesis that the aging mammalian heart and brain might have increased vulnerability to acute stress, DNA fragmentation was studied after hypoxia-reoygenation in young adult (6 months) and old (22-24 months) F344 rats. Heart and brain tissue were examined at the following time points: 30, 60, or 90 min o...

journal_title：Mechanisms of ageing and development

authors： Azhar G,Liu L,Zhang X,Wei JY

更新日期：1999-12-07 00:00:00

abstract：:Type I and II macrophage scavenger receptors are implicated in the pathologic deposition of cholesterol during the atherogenesis. There is a charged collagen structure of type I and II receptors identified as a ligand binding domain, which can recognize a wide range of negatively charged macromolecules including oxidi...

journal_title：Mechanisms of ageing and development

authors： Shirai H,Murakami T,Yamada Y,Doi T,Hamakubo T,Kodama T

更新日期：1999-11-01 00:00:00

abstract：:In an attempt to understand the molecular mechanisms of age-dependent degenerative alteration in human periodontal tissues, we examined mRNA level and DNA methylation of collagen alpha1(I) gene. Using healthy periodontal ligament tissues from humans aged 9-76 years, we found that the collagen alpha1(I) mRNA level decr...

journal_title：Mechanisms of ageing and development

authors： Takatsu M,Uyeno S,Komura J,Watanabe M,Ono T

更新日期：1999-10-01 00:00:00

abstract：:The present study investigated whether age-related changes in the production of Th1 and Th2 cytokines by human T cells might be linked to altered frequencies of naive (CD45RA+) and memory (CD45RO+) T cell subsets. T cells from healthy elderly humans (n = 32) stimulated with anti-CD3epsilon monoclonal antibody OKT3 plu...

journal_title：Mechanisms of ageing and development

authors： Karanfilov CI,Liu B,Fox CC,Lakshmanan RR,Whisler RL

更新日期：1999-08-30 00:00:00

abstract：:In this study, we showed that in flies kept for 2 weeks at 1 (terrestrial gravity), 3 or 5 x g (hypergravity, HG) before transfer to 1 x g, resistance to heat remained higher in HG flies for several weeks after the transfer. The measurement of heat shock protein 70 (hsp70) indicated no induction of the protein in HG, ...

journal_title：Mechanisms of ageing and development

authors： Minois N,Guinaudy MJ,Payre F,Le Bourg E

更新日期：1999-06-01 00:00:00

abstract：:Changes in the T-lymphocyte compartment represent the most critical component of immunological aging. Recent studies have demonstrated that the age-related decline in T-cell-mediated immunity is a multifactorial phenomenon affecting T-cell subset composition as well as several proximal events such as protein tyrosine ...

journal_title：Mechanisms of ageing and development

authors： Chakravarti B,Abraham GN

更新日期：1999-05-17 00:00:00

abstract：:Interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) concentrations were measured in livers of young-adult and old rats administered carbon tetrachloride or vehicle. IL-1beta levels were higher and IL-6 levels were lower in old rats than in young-adult rats. Carbon tetrachlor...

journal_title：Mechanisms of ageing and development

authors： Rikans LE,DeCicco LA,Hornbrook KR,Yamano T

更新日期：1999-05-03 00:00:00

abstract：:The responses of the left and right ventricles (LV and RV) to physical conditioning in cold (25 degrees C) and thermoneutral temperatures (35 degrees C), with special reference to lactate dehydrogenase (LDH) and its isoenzyme profile, were studied in the 2-month (young)- and 12-month (middle-aged)-old rats. Moderate h...

journal_title：Mechanisms of ageing and development

authors： Prathima S,Devi SA

更新日期：1999-04-01 00:00:00

abstract：:Protein carbonyls were studied in aging and exercise by immunoblot followed by one- or two-dimensional polyacrylamide gel electrophoresis using antibodies against 2,4-dinitrophenylhydrazones. Proteins of rat kidneys exhibited significant age-related increase in the amount of carbonyl while those of the brain and liver...

journal_title：Mechanisms of ageing and development

authors： Goto S,Nakamura A,Radak Z,Nakamoto H,Takahashi R,Yasuda K,Sakurai Y,Ishii N

更新日期：1999-03-15 00:00:00

abstract：:Taurine is a sulfur amino acid that is present in high concentration in mammalian tissues and previously has been reported to decline in a number of tissues with advancing age. The aims of the present study were to examine: (1) the effects of dietary taurine supplementation; (2) the effects of taurine-free diets; (3) ...

journal_title：Mechanisms of ageing and development

authors： Dawson R Jr,Liu S,Eppler B,Patterson T

更新日期：1999-02-01 00:00:00

abstract：:In agreement with previous studies we show an age-related increase in serum levels of hyaluronic acid in healthy individuals. Levels in Down's syndrome persons were slightly higher, especially in those with hypothyroidism. This provides further evidence that the normal ageing process is accelerated in Down's syndrome....

journal_title：Mechanisms of ageing and development

authors： Hutchin T,Martin L,Prasher V,Sinclair AJ

更新日期：1998-12-01 00:00:00

abstract：:The study aimed to evaluate the effect of age on the activity of the cytochrome P450-dependent monooxygenase system and on cellular respiration processes in Wistar rats aged 0.5, 1, 2, 4, 8, 12, 20, and 28 months. The following parameters were determined: cytochrome P450 content, cytochrome b5 content, NADPH-cytochrom...

journal_title：Mechanisms of ageing and development

authors： Plewka A,Kamiński M,Plewka D

更新日期：1998-11-16 00:00:00

abstract：:A 3'-->5' exonuclease found in rat liver excises mispaired nucleotides at the 3'-hydroxyl end of primer chains such as poly dA-d(T9-C). Consequently, the priming activity of the chain from which the mispaired base was cut is greatly increased during DNA synthesis. These results suggest that the 3'-->5' exonuclease act...

journal_title：Mechanisms of ageing and development

authors： Taguchi T,Fukuda M,Toda T,Ohashi M

更新日期：1998-09-15 00:00:00

abstract：:Food restriction is known to ameliorate many of the adverse physiologic effects of age. In this study, we have examined the effect of food restriction on the induction of the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase (PEPCK) in liver and kidney following a 12-h fasting period in young (6 month) and old (...

journal_title：Mechanisms of ageing and development

authors： Van Remmen H,Ward WF

更新日期：1998-09-01 00:00:00

abstract：:An established senescence-accelerated model mouse strain, SAMP8, shows the deterioration of learning and memory compared with a normal control strain, SAMR1. D-Serine binds to strychnine-insensitive glycine binding sites of the N-methyl-D-aspartate (NMDA) receptor complex, and enhances glutamate binding to the recepto...

journal_title：Mechanisms of ageing and development

authors： Nagata Y,Uehara T,Kitamura Y,Nomura Y,Horiike K

更新日期：1998-08-14 00:00:00

abstract：:Healthy elderly (80+/-5 years) with different nutritional status were compared to young healthy adults (25+/-5 years) to quantify the relative influences of aging and nutrition on immune response. Aged persons, without alteration of their nutritional status, had lower CD3+, CD8+, and CD45RA+ as well as higher CD2+CD3-...

journal_title：Mechanisms of ageing and development

authors： Mazari L,Lesourd BM

更新日期：1998-08-01 00:00:00

abstract：:The effect of aging on patterns of food intake and nutrient selection was investigated using a longitudinal study. Male (n = 10) and female (n = 10) Wistar-Lou rats from 4 to 28 months of age were repeatedly submitted to a macronutrient self-selection (S-S) regimen while controls were maintained under a standard chow ...

journal_title：Mechanisms of ageing and development

authors： Veyrat-Durebex C,Boghossian S,Alliot J

更新日期：1998-07-15 00:00:00

abstract：:The purpose of this research is to determine possible causes and mechanisms involved in the age-associated decline in mitochondrial activity. We have focused on cytochrome c oxidase because it is comprised of both nuclear and mitochondrial-encoded subunits and may provide some insight into the coordination of the two ...

journal_title：Mechanisms of ageing and development

authors： Hudson EK,Tsuchiya N,Hansford RG

更新日期：1998-06-15 00:00:00

abstract：:Circulating antibodies in the sera of normal, healthy humans of different age groups to native DNA (nDNA) and reactive oxygen species modified DNA (ROS-DNA) was studied by competition ELISA. Sera from the young population (< 50 years of age) showed negligible levels of anti-DNA antibodies. In contrast, anti-DNA antibo...

journal_title：Mechanisms of ageing and development

authors： Ashok BT,Ali R

更新日期：1998-06-01 00:00:00

abstract：:The present study examined the effect of repeated vaccination and of dehydroepiandrosterone (DHEA) treatment on the immune response to influenza vaccine in elderly subjects. Seventy-one elderly volunteers, aged 61-89 years, enrolled in a prospective randomized, double-blind study to receive either DHEA (50 mg qd p.o. ...

journal_title：Mechanisms of ageing and development

authors： Ben-Yehuda A,Danenberg HD,Zakay-Rones Z,Gross DJ,Friedman G

更新日期：1998-05-15 00:00:00

abstract：:The effects of aging on the activation of the cytoplasmic tyrosine protein kinase p56(lck) have been investigated in PBL from adult and elderly subjects upon activation with mitogens or different co-stimuli. Results show that the amount and phosphorylation of p56(lck) are reduced in PBL from elderly as compared to adu...

journal_title：Mechanisms of ageing and development

authors： Guidi L,Antico L,Bartoloni C,Costanzo M,Errani A,Tricerri A,Vangeli M,Doria G,Gatta L,Goso C,Mancino L,Frasca D

更新日期：1998-05-15 00:00:00

abstract：:We studied the pattern of lectins binding by liver lysosomal proteins from rats between 18 days of gestation and 72 weeks of age. An analysis of the carbohydrate structure was carried out after an electrophoresis and blotting, followed by a very sensitive detection system with highly specific digoxigenin-labelled lect...

journal_title：Mechanisms of ageing and development

authors： Lityńska A,Przybyło M

更新日期：1998-05-01 00:00:00

abstract：:This study aimed to assess platelets as a possible model for screening the accumulation of mitochondrial DNA mutations, particularly during normal ageing. For this purpose we isolated platelets from young and old donors selected by lack of systemic and haematological diseases. We studied the accumulation of a particul...

journal_title：Mechanisms of ageing and development

authors： Biagini G,Pallotti F,Carraro S,Sgarbi G,Pich MM,Lenaz G,Anzivino F,Gualandi G,Xin D

更新日期：1998-04-01 00:00:00

abstract：:Endothelin (ET) is a powerful vasopressor agent that is activated in a number of pathophysiologic states where renal perfusion is reduced. Since renal vasoconstriction occurs as part of renal aging, we investigated the possibility that ET may be activated in the old kidney. These experiments involved acutely blocking ...

journal_title：Mechanisms of ageing and development

authors： Greenfeld Z,Engels K,Samsell L,Baylis C

更新日期：1998-03-16 00:00:00

abstract：:The effects of interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), and lipopolysaccharide (LPS) on the productions of nitric oxide (NO) and cGMP by cultured articular chondrocyte (AC) monolayers from 1-, 8- and 18-month old male Wistar rats were studied. It was found that basal NO and cGMP productions decre...

journal_title：Mechanisms of ageing and development

authors： Khatib AM,Siegfried G,Messai H,Quintero M,Barbara A,Mitrovic RD

更新日期：1998-03-16 00:00:00

abstract：:Food restriction increases maximal life span in rodents. Male rats that exercise in voluntary running wheels do not have an increase in maximal longevity despite a relative caloric deficit. In contrast, sedentary rats that are food restricted so as to cause the same caloric deficit have an extension of maximal longevi...

journal_title：Mechanisms of ageing and development

authors： Holloszy JO

更新日期：1998-02-16 00:00:00